Phenotypic Analyses of Genetically Modified Mice for BMP Receptors

Jingwen Yang; Yuji Mishina

doi:10.1007/978-1-4939-8904-1_13

Phenotypic Analyses of Genetically Modified Mice for BMP Receptors

Methods Mol Biol. 2019:1891:179-189. doi: 10.1007/978-1-4939-8904-1_13.

Authors

Jingwen Yang^{1

2}, Yuji Mishina³

Affiliations

¹ Department of Biologic and Materials Sciences, School of Dentistry, University of Michigan, Ann Arbor, MI, USA.
² State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory for Oral Biomedicine of Ministry of Education (KLOBM), School and Hospital of Stomatology, Wuhan University, Wuhan, China.
³ Department of Biologic and Materials Sciences, School of Dentistry, University of Michigan, Ann Arbor, MI, USA. mishina@umich.edu.

Abstract

BMPs play important roles in the development, disease, and regeneration of many tissues. Genetically modified mice with altered BMP receptor genes are particularly informative for clarifying the role of BMP signaling. In this chapter, we introduce several selected protocols for in vivo functional characterization of BMP receptors in genetically modified mice, including immunohistochemistry of BMP downstream signaling (P-Smad1/5/9 or others), histological analysis, whole-mount skeletal staining for cartilage and bone tissues, and whole-mount cartilage staining.

Keywords: BMP receptors; Histological analysis; Immunohistochemistry; Transgenic; Whole-mount cartilage staining; Whole-mount skeletal staining.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Biomarkers
Bone Morphogenetic Protein Receptors / genetics*
Bone Morphogenetic Protein Receptors / metabolism
Genotype
Immunohistochemistry
Mice
Mice, Transgenic*
Phenotype*
Smad Proteins / genetics
Smad Proteins / metabolism

Substances

Biomarkers
Smad Proteins
Bone Morphogenetic Protein Receptors

Grants and funding

R01 DE020843/DE/NIDCR NIH HHS/United States