In Vitro and In Vivo Osteogenic Differentiation of Human Adipose-Derived Stromal Cells

Methods Mol Biol. 2019:1891:9-18. doi: 10.1007/978-1-4939-8904-1_2.

Abstract

Adipose-derived stromal cells (ASCs) are a promising population of cells that may be useful for the regeneration of human tissue defects. ASCs are capable of forming bone tissue in vitro and in vivo. Further work is required to determine the optimal conditions that will allow human ASCs to regenerate tissue in clinically significant tissue defects. Here we present three experimental protocols that are indispensable for the study of ASC osteogenic activity.

Keywords: Adipose-derived stromal cell; Bone defect; Calvarial defect; Osteogenesis; Osteogenic differentiation.

MeSH terms

  • Adipose Tissue / cytology*
  • Adipose Tissue / metabolism
  • Antigens, Surface / metabolism
  • Biomarkers
  • Bone Regeneration
  • Bone and Bones / injuries
  • Cell Differentiation*
  • Humans
  • Osteogenesis*
  • Stromal Cells / metabolism*

Substances

  • Antigens, Surface
  • Biomarkers