We describe the procedure for inoculating overnight (starter) cultures of E. coli from a single colony, along with considerations for growing larger cultures. We also include two methods for monitoring the number of cells per unit volume (density) of liquid cultures using a spectrophotometer and a hemacytometer or "count slide." © 2018 by John Wiley & Sons, Inc.
Keywords: Escherichia coli K-12; aeration; cell counting; hemacytometer; spectrophotometer.
© 2018 John Wiley & Sons, Inc.