Growth of E. coli in Liquid Medium

Curr Protoc Mol Biol. 2019 Jan;125(1):e81. doi: 10.1002/cpmb.81. Epub 2018 Nov 9.

Abstract

We describe the procedure for inoculating overnight (starter) cultures of E. coli from a single colony, along with considerations for growing larger cultures. We also include two methods for monitoring the number of cells per unit volume (density) of liquid cultures using a spectrophotometer and a hemacytometer or "count slide." © 2018 by John Wiley & Sons, Inc.

Keywords: Escherichia coli K-12; aeration; cell counting; hemacytometer; spectrophotometer.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Colony Count, Microbial / instrumentation
  • Colony Count, Microbial / methods*
  • Culture Media / chemistry*
  • Culture Media / metabolism
  • Escherichia coli / chemistry
  • Escherichia coli / growth & development*
  • Escherichia coli / metabolism
  • Spectrophotometry / methods*

Substances

  • Culture Media