Colloidal Fluorophore Aggregates for the Selective Detection of Albumins in Solution and on Electrophoresis Gels

Jihee Park; Youngmi Kim

doi:10.1002/cbic.201800608

Colloidal Fluorophore Aggregates for the Selective Detection of Albumins in Solution and on Electrophoresis Gels

Chembiochem. 2019 Feb 1;20(3):350-354. doi: 10.1002/cbic.201800608. Epub 2018 Dec 27.

Authors

Jihee Park¹, Youngmi Kim¹

Affiliation

¹ Department of Chemistry, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul, 02447, Republic of Korea.

PMID: 30411837
DOI: 10.1002/cbic.201800608

Abstract

We report a fluorescent probe that is highly sensitive and selective for serum albumins. Signal transduction results from the disassembly of fluorescence-quenched aggregates upon binding to human serum albumin (HSA). The probe offers a rapid (≤2 s) fluorometric assay of HSA in phosphate-buffered saline, urine, or blood serum with a high signal-to-noise ratio. It is also useful as a wash-free prestaining reagent for detecting HSA on electrophoresis gels.

Keywords: aggregation; fluorescence; imaging agents; proteins; self-assembly.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Colloids / chemistry
Electrophoresis, Capillary
Fluorescent Dyes / chemistry*
Gels / chemistry
Humans
Models, Molecular
Molecular Structure
Serum Albumin / analysis*
Solutions
Spectrometry, Fluorescence

Substances

Colloids
Fluorescent Dyes
Gels
Serum Albumin
Solutions