Abstract
For the quantification of mutated genes at greatly varied concentrations in body fluids, nucleic acid amplification strategies are often challenged by limited dynamic linear ranges. The proposed metal stable isotope detection strategy demonstrates five orders of linear range and a lower attomol detection limit, showing promising potential in clinical diagnosis.
MeSH terms
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Biosensing Techniques / methods
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DNA / analysis*
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DNA / blood
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DNA Probes / chemistry
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DNA Probes / genetics
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DNA, Viral / analysis
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DNA, Viral / blood
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Gold / chemistry*
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HIV Infections / blood
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HIV-1 / isolation & purification
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Humans
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Isotope Labeling / methods
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Limit of Detection
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Mass Spectrometry / methods*
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Metal Nanoparticles / chemistry*
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Nucleic Acid Amplification Techniques / methods*
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Nucleic Acid Hybridization / methods
Substances
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DNA Probes
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DNA, Viral
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Gold
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DNA