Abstract
A novel EGFR-targeting, thermal-sensitive multifunctional liposome (TSML) was developed based on manganese-doped magnetism-engineered iron oxide nanoparticles (MnMEIOs) and gold nanorods (AuNRs) for efficient photothermal therapy and magnetic resonance (MR) imaging. An Erbitux-conjugated TSML (Erb-TSML) was encapsulated with doxorubicin and gold nanorods conjugated with manganese-doped magnetism-engineered iron oxide nanoparticles, for theranostic applications of EGFR-positive tumors. The Erb-TSML selectively targeted EGFR-positive tumors and promoted tumor destruction by laser activation. Using confocal microscopy, MR and optical imaging, we demonstrated that Erb-TSML specifically bound to A431 tumor cells. No signs of major morphological damages to the normal tissues were observed in mice treated with Erb-TSML and laser, indicating this theranostic platform protected heart against doxorubicin-induced toxicity to normal tissues. These results indicate that the Erb-TSML may be a promising diagnostic and therapeutic platform for EGFR-overexpressing tumors.
Keywords:
AuNR@MnMEIO; Doxorubicin; EGFR; Erbitux; MRI; TSML.
Copyright © 2018 Elsevier Inc. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibiotics, Antineoplastic / administration & dosage
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Antibiotics, Antineoplastic / chemistry
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Antibiotics, Antineoplastic / pharmacology
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Antineoplastic Agents, Immunological / administration & dosage
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Antineoplastic Agents, Immunological / chemistry
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Antineoplastic Agents, Immunological / pharmacology
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Apoptosis
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Cell Proliferation
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Cetuximab / administration & dosage
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Cetuximab / chemistry
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Cetuximab / pharmacology
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Doxorubicin / administration & dosage
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Doxorubicin / chemistry
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Doxorubicin / pharmacology
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ErbB Receptors / antagonists & inhibitors
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Gold / chemistry
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Humans
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Immunoconjugates / administration & dosage
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Immunoconjugates / chemistry
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Immunoconjugates / pharmacology*
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Lasers*
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Liposomes / administration & dosage*
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Liposomes / chemistry
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Lung Neoplasms / drug therapy*
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Lung Neoplasms / metabolism
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Lung Neoplasms / pathology
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Magnetite Nanoparticles / administration & dosage*
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Magnetite Nanoparticles / chemistry
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Mice
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Mice, Inbred BALB C
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Mice, Nude
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Nanotubes / chemistry*
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Optical Imaging
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Tumor Cells, Cultured
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Xenograft Model Antitumor Assays
Substances
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Antibiotics, Antineoplastic
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Antineoplastic Agents, Immunological
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Immunoconjugates
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Liposomes
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Magnetite Nanoparticles
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Gold
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Doxorubicin
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EGFR protein, human
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ErbB Receptors
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Cetuximab