Effect of a novel antifungal peptide P852 on cell morphology and membrane permeability of Fusarium oxysporum

Yuzhu Han; Jianjun Zhao; Bao Zhang; Qian Shen; Qingmao Shang; Pinglan Li

doi:10.1016/j.bbamem.2018.10.018

Effect of a novel antifungal peptide P852 on cell morphology and membrane permeability of Fusarium oxysporum

Biochim Biophys Acta Biomembr. 2019 Feb 1;1861(2):532-539. doi: 10.1016/j.bbamem.2018.10.018. Epub 2018 Oct 31.

Authors

Yuzhu Han¹, Jianjun Zhao², Bao Zhang³, Qian Shen⁴, Qingmao Shang⁵, Pinglan Li⁶

Affiliations

¹ Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China; College of Animal Science, Southwest University, Chongqing, China.
² College of Animal Science, Southwest University, Chongqing, China.
³ Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China.
⁴ Department of Microbiology, Ohio State University, Columbus, OH, United States.
⁵ Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China.
⁶ Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China. Electronic address: lipinglanhyz@126.com.

PMID: 30391317
DOI: 10.1016/j.bbamem.2018.10.018

Abstract

P852, a novel cyclic peptide isolated from Bacillus amyloliquefaciens L-H15, showed potent antifungal activity against several major plant fungal pathogens including Fusarium oxysporum. To elucidate the antifungal mechanism, the impact of P852 on the cell morphology and membrane permeabilization of F. oxysporum was studied. By applying electron microscopy and fluorescent techniques, we showed that P852 treatment caused the morphological change of F. oxysporum cells and disrupted its cell structure, including formation of blebs, broken hyphae, deformation of membrane, intracellular organization disruption, pore formation, and cell lysis. Our findings provide insights into the mode of action of P852, which laying a foundation to develop P852 as a novel antifungal agent to control plant fungal pathogens.

Keywords: Antifungal peptide; Cell morphology; Fusarium oxysporum; Membrane permeabilization.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amphotericin B / pharmacology
Antifungal Agents / pharmacology*
Cell Membrane / drug effects
Cell Membrane / metabolism
Cell Membrane Permeability / drug effects*
DNA, Fungal / metabolism
Fluorescence Polarization
Fusarium / cytology*
Fusarium / drug effects
Fusarium / ultrastructure
Hyphae / cytology
Hyphae / drug effects
Hyphae / ultrastructure
Inhibitory Concentration 50
Kinetics
Membrane Fluidity / drug effects
Microbial Sensitivity Tests
Organic Chemicals / metabolism
Peptides / pharmacology*

Substances

Antifungal Agents
DNA, Fungal
Organic Chemicals
Peptides
SYTOX Green
Amphotericin B