Isolation and functional characterization of SUCROSE SYNTHASE 1 and SUCROSE TRANSPORTER 2 promoters from ramie (Boehmeria nivea L. Gaudich)

Pingan Guo; Yancheng Zheng; Yanyan He; Lijun Liu; Bo Wang; Dingxiang Peng

doi:10.1016/j.gene.2018.10.081

Isolation and functional characterization of SUCROSE SYNTHASE 1 and SUCROSE TRANSPORTER 2 promoters from ramie (Boehmeria nivea L. Gaudich)

Gene. 2019 Feb 15:685:114-124. doi: 10.1016/j.gene.2018.10.081. Epub 2018 Oct 30.

Authors

Pingan Guo¹, Yancheng Zheng¹, Yanyan He², Lijun Liu¹, Bo Wang³, Dingxiang Peng⁴

Affiliations

¹ MOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science and Technology, Huazhong Agricultural University, #1 Shizishan Street, Hongshan District, Wuhan 430070, Hubei Province, China.
² Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
³ MOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science and Technology, Huazhong Agricultural University, #1 Shizishan Street, Hongshan District, Wuhan 430070, Hubei Province, China. Electronic address: wangbo@mail.hzau.edu.cn.
⁴ MOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science and Technology, Huazhong Agricultural University, #1 Shizishan Street, Hongshan District, Wuhan 430070, Hubei Province, China. Electronic address: pdxiang@mail.hzau.edu.cn.

PMID: 30385302
DOI: 10.1016/j.gene.2018.10.081

Abstract

Sucrose synthase and sucrose transporter are involved in sucrose metabolism and partitioning of photosynthetic products, respectively. In this study, we cloned SUCROSE SYNTHASE 1 and SUCROSE TRANSPORTER 2 genes from ramie. Real-time quantitative PCR revealed that BnSUS1 and BnSUT2 were widely expressed in the analyzed tissues. Subsequently, the two promoters of BnSUS1 and BnSUT2 were isolated and truncated. The two promoters and their truncated fragments were fused GUS to transform into Arabidopsis. GUS staining showed that BnSUS1_pro-1690 and BnSUS1_pro-1420 had vascular specificity in cotyledons and mature leaves while BnSUT2_pro-2239, BnSUT2_pro-1681, BnSUT2_pro-1199 and BnSUT2_pro-618 had a constitutive function in seedlings and mature organs. Notably, the activity of BnSUT2_pro-2239 and its fragments (except that of BnSUT2_pro-231) are strongly induced by mechanical wounding. Moreover, BnSUS1_pro-1051 and BnSUS1_pro-485 are sensitive to CuSO₄ treatment while BnSUT2_pro-2239 and BnSUT2_pro-1681 are sensitive to PEG and ABA treatments, respectively. Our findings will provide the foundation for deciphering the functions of BnSUS1 and BnSUT2, and also expand the promoter library to provide more options for plant genetic engineering.

Keywords: Promoter; Ramie; SUCROSE SYNTHASE 1; SUCROSE TRANSPORTER 2.

MeSH terms

Arabidopsis / genetics
Boehmeria / genetics*
Cloning, Molecular*
Exons
Gene Expression Regulation, Plant
Glucosyltransferases / genetics*
Introns
Membrane Transport Proteins / genetics*
Phenotype
Phylogeny
Plant Proteins / genetics*
Plants, Genetically Modified
Promoter Regions, Genetic*

Substances

Membrane Transport Proteins
Plant Proteins
sucrose transport protein, plant
Glucosyltransferases
sucrose synthase