Objective To explore the relationship between resistin and PPARγ and to investigate the pro-inflammatory functions of bovine resistin. Methods Bovine alveolar macrophages (BAMs) were incubated with 100 ng/mL bovine-resistin for 0, 1.5, 3, 6, 12, 24 hours, respectively. The mRNA expression levels of PPARγ, NF-κB, and resistin were tested by qRT-PCR, the protein expression levels of PPARγ and NF-κB were analyzed by Western blot analysis, and the levels of pro-inflammatory cytokines (IL-1β and TNF-α) in the supernatant were measured by ELISA. Results After BAMs were treated with resistin for 1.5 hours, the level of PPARγ of BAMs was significantly reduced and there was a time dependent effect. Meanwhile, the mRNA levels of NF-κB and resistin in BAMs increased significantly since induced by bovine resistin for 6 hours and to peak at 12 hours. The protein expression of PPARγ in BAMs decreased significantly after incubating with bovine-resistin for 12 hours, while the expression of NF-κB increased significantly at 12 hours. Both IL-1β and TNF-α increased in a time-dependent manner after 1.5 hours. Conclusion Bovine-resistin might induce BAMs producing pro-inflammatory cytokines such as IL-1β and TNF-α via inhibiting PPARγ whereas activating the NF-κB mediated pathway.