In this study we performed a comparative gene expression analysis of carotid arteries in the area of atherosclerotic plaques and healthy internal mammary arteries of patients with advanced atherosclerosis by using microarray HumanHT-12 BeadChip ("Illumina"). The most down-regulated genes were APOD, FABP4, CIDEC and FOSB, and up-regulated gene was SPP1 (|FC|>64; pFDR<0.05). The majority of differentially expressed genes were down-regulated in advanced atherosclerotic plaques. Unexpectedly, genes involved in immune and inflammatory responses were down-regulated in advanced atherosclerotic plaques to compare with the healthy arteries (arachidonic acid metabolism, cytokine-cytokine receptor interaction, NOD-like receptor signaling pathway, Jak-STAT signaling pathway, TNF signaling pathway). "Cellular response to metal ion" (metallothioneins) and "Extracellular matrix organization" were the most significant Gene ontology terms among the down- and up-regulated genes, respectively.
S ispol'zovaniem mikrochipov HumanHT-12 BeadChip (“Illumina”, SShA) proveden sravnitel'nyĭ polnogenomnyĭ analiz ékspressii genov v kletkakh sonnykh arteriĭ, porazhennykh aterosklerozom, i kletkakh intaktnykh vnutrennikh grudnykh arteriĭ u patsientov s klinicheski vyrazhennym aterosklerozom na pozdnikh stadiiakh patologicheskogo protsessa. Sushchestvennoe snizhenie urovnia ékspressii v kletkakh sonnykh arteriĭ, porazhennykh aterosklerozom, po sravneniiu s intaktnymi vnutrennimi grudnymi arteriiami, vyiavleno dlia genov APOD, FABP4, CIDEC i FOSB, a uvelichenie ékspressii – dlia gena SPP1 (|FC|>64; pFDR<0,05). V kletkakh ateroskleroticheski porazhennykh arteriĭ po sravneniiu s intaktnymi sosudami vyiavleno preobladanie doli genov so snizhennoĭ funktsional'noĭ aktivnost'iu. V chastnosti, vyiavleno snizhenie ékspressii genov immunovospalitel'nogo otveta (metabolizm arakhidonovoĭ kisloty, vzaimodeĭstvie “tsitokin-tsitokinovyĭ retseptor”, signal'nye puti NOD-podobnykh retseptorov, Jak-STAT i TNF). Naibolee znachimymi biologicheskimi protsessami, v kotorykh uchastvuiut belkovye produkty genov so snizheniem ékspressii v ateroskleroticheski porazhennykh sonnykh arteriiakh po sravneniiu s intaktnymi vnutrennimi grudnymi arteriiami, iavliaiutsia otvety kletki na deĭstvie ionov metallov (metallotioneiny), a dlia belkovykh produktov genov s uvelicheniem ékspressii v ateroskleroticheski izmenennykh arteriiakh - organizatsiia vnekletochnogo matriksa.
Keywords: carotid atherosclerosis; gene expression; microarrays; vascular tissues.