Abstract
FLIRT (fast local infrared thermogenetics) is a microscopy-based technology to locally and reversibly manipulate protein function while simultaneously monitoring the effects in vivo. FLIRT locally inactivates fast-acting temperature-sensitive mutant proteins. We demonstrate that FLIRT can control temperature-sensitive proteins required for cell division, Delta-Notch cell fate signaling, and germline structure in Caenorhabditis elegans with cell-specific and even subcellular precision.
Publication types
-
Research Support, N.I.H., Extramural
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Caenorhabditis elegans / genetics
-
Caenorhabditis elegans / physiology*
-
Caenorhabditis elegans / radiation effects
-
Caenorhabditis elegans Proteins / genetics
-
Caenorhabditis elegans Proteins / metabolism*
-
Cell Differentiation
-
Embryo, Nonmammalian / cytology
-
Embryo, Nonmammalian / physiology
-
Gene Expression Regulation
-
Genetic Techniques / instrumentation*
-
Germ Cells
-
Infrared Rays*
-
Microscopy
-
Molecular Imaging / methods*
-
Mutation*
-
Receptors, Notch
-
Signal Transduction
-
Temperature*
Substances
-
Caenorhabditis elegans Proteins
-
Receptors, Notch