The online monitoring and assessment of thallium stress using oxygen consumption rate and carbon dioxide excretion rate of zebrafish (Danio rerio)

Abstract

An online monitoring and assessment system of metabolism to measure oxygen consumption rate (OC) and carbon dioxide (CO₂) excretion rate (CR) of zebrafish (Danio rerio) was used to illustrate changes in stressful states in 15 days' (360 h) 0.1 μg/L Tl exposure. Tl had a significant inhibition on zebrafish OC and CR (p < 0.01). OC was more suitable for Tl stress assessment than CR, considering that the OC response was more stable and discernible from the control comparing with CR. However, CR is a suitable alternative to characterize toxic effects on different metabolic substrates. Both OC and CR were integrated to present the respiratory quotient (RQ) analysis. RQ was efficient in differentiating between CO₂ produced by respiration in the control group (RQ less than 0.7) and CO₂ used for urination or stored in tissues after Tl exposure (some RQs larger than 1.0). Circadian rhythm was observed in RC and CR in the controls and persisted in 0.1 μg/L Tl treatments. The rhythm was relatively more disordered in CR. OC and CR would be suitable for indicating physiological stress in the online system as sensitive physiological indices.

Keywords: Carbon dioxide excretion rate; Circadian rhythm; Online monitoring and assessment; Oxygen consumption rate; Zebrafish (Danio rerio).