Establishing methods for isolation of stem cells from human exfoliated deciduous from carious deciduous teeth

Masagus Zainuri; Ratih Rinendya Putri; Endang W Bachtiar

doi:10.1556/1646.10.2018.06

Establishing methods for isolation of stem cells from human exfoliated deciduous from carious deciduous teeth

Interv Med Appl Sci. 2018 Mar;10(1):33-37. doi: 10.1556/1646.10.2018.06.

Authors

Masagus Zainuri¹, Ratih Rinendya Putri¹, Endang W Bachtiar²

Affiliations

¹ Center for Research and Development of Biomedical and Basic Health Technology, National Institute Health Research and Development, Ministry of Health Republic of Indonesia, Jakarta, Indonesia.
² Faculty of Dentistry, Department of Oral Biology, Oral Sciences Research Center, Universitas Indonesia, Jakarta, Indonesia.

Abstract

Aim: This study aims to establish the isolation method of stem cells from pulp tissue of carious deciduous teeth.

Methods: The teeth were soaked in 1% povidone-iodine solution for about 1 min followed by washing in PBS with 1% antibiotic-antimycotic thrice. Dental pulp tissue was removed by extirpation, and then cultivated in the culture medium. Characterization of mesenchymal stem cell (MSC) was carried out using human MSC analysis kit with positive markers CD90, CD73, and CD105, but negative for expressions of CD45, CD34, CD11b, CD19, and HLA-DR. Differentiation capacity of stem cells from human exfoliated deciduous (SHED) was determined by staining with Alizarin S, Alcian Blue, and Oil Red O.

Results: There is no contamination after 3 days of culture. SHED derived from dental pulp were expressions of 99.2% of positive marker and 0.3% of the negative marker. At passage 5, SHED was differentiated into osteocyte, chondrocyte, and adipocyte types of cells in the induction medium.

Conclusion: SHED derived from carious deciduous teeth can be used as a source of stem cell for regenerative medicine.

Keywords: Alcian Blue; Alizarin; Oil Red O; dental pulp stem cell (DPSC); stem cells from human exfoliated deciduous (SHED).

Grants and funding

Funding sources: This study was funded by the Center for Research and Development of Biomedical and Basic Health Technology, National Institute Health Research and Development, Ministry of the Health Republic of Indonesia (2016).