Sarcolemmal membrane excitability during repeated intermittent maximal voluntary contractions

Abstract

New findings: What is the central question of this study? Is impaired membrane excitability reflected by an increase or by a decrease in M-wave amplitude? What is the main finding and its importance? The magnitude of the M-wave first and second phases changed in completely different ways during intermittent maximal voluntary contractions, leading to the counterintuitive conclusion that it is an increase (and not a decrease) of the M-wave first phase that reflects impaired membrane excitability.

Abstract: The study was undertaken to investigate separately the changes in the first and second phases of the muscle compound action potential (M-wave) during 4 min of intermittent maximal voluntary contractions (MVCs) of the quadriceps. M-waves were evoked by supramaximal single electrical stimulation to the femoral nerve delivered in the resting periods between 48 successive MVCs of 3 s. The amplitude, duration and area of the M-wave first and second phases were measured separately, together with muscle conduction velocity and MVC force. During the intermittent MVCs, the amplitude of the M-wave first phase increased uninterruptedly for the first 3 min (12-16%, P < 0.05) and stabilized thereafter, whereas the second phase initially increased for 55-75 s (11-22%, P < 0.05), but decreased subsequently. The enlargement of the first phase occurred in parallel with an increase in its duration, and concomitantly with a decline in conduction velocity (maximal cross-correlations, 0.89-0.97; time lag, 0 s). Also, a significant temporal association was found between the amplitude of the first phase and MVC force (time lag, 0 s; maximal cross-correlations, 0.85-0.97). Conversely, there was no temporal association between the second phase amplitude and conduction velocity or MVC force (time lag, 73-117 s; maximal cross-correlations, 0.65-0.77). It is concluded that the enlargement of the M-wave first phase is the electrical manifestation of impaired muscle membrane excitability. The results highlight the importance of independently analysing the first and second phases, as only the first phase can be used reliably to detect changes in membrane excitability, while the second might be affected by muscle architecture.