Bacterial biopolymer (polyhydroxyalkanoate) production from low-cost sustainable sources

Abstract

Twenty-six different bacterial strains were isolated from samples taken from different locations Dammam, Saudi Arabia, for screening of their polyhydroxyalkanoate (PHA) production capability. The initial screening was conducted by staining with Sudan Black B and Nile Red, followed by examination under fluorescence and electron microscopes to characterize PHA granule formation. The PHA-producing bacterial isolates were identified using 16S rRNA gene analyses; the most potent bacterial strain was identified as Pseudomonas sp. strain-P(16). The PHA production capability of this strain in the presence of different low-cost carbon sources, such as rice bran, dates, and soy molasses, was analyzed. PHA production in the presence of rice bran, dates, and soy molasses was 90.9%, 82.6%, and 91.6%, respectively.

Keywords: PHA yield; dates; fluorescence microscopy; low-cost carbon source.