Optofluidic detection of Zika nucleic acid and protein biomarkers using multimode interference multiplexing

Alexandra Stambaugh; Joshua W Parks; Matthew A Stott; Gopikrishnan G Meena; Aaron R Hawkins; Holger Schmidt

doi:10.1364/BOE.9.003725

Optofluidic detection of Zika nucleic acid and protein biomarkers using multimode interference multiplexing

Biomed Opt Express. 2018 Jul 16;9(8):3725-3730. doi: 10.1364/BOE.9.003725. eCollection 2018 Aug 1.

Authors

Alexandra Stambaugh¹, Joshua W Parks¹, Matthew A Stott², Gopikrishnan G Meena¹, Aaron R Hawkins², Holger Schmidt¹

Affiliations

¹ School of Engineering, University of California, Santa Cruz, 1156 High St., Santa Cruz, CA 95064, USA.
² ECEn Department, Brigham Young University, 459 Clyde Building, Provo, UT 84602, USA.

Abstract

The recent massive Zika virus (ZIKV) outbreak illustrates the need for rapid and specific diagnostic techniques. Detecting ZIKV in biological samples poses unique problems: antibody detection of ZIKV is insufficient due to cross-reactivity of Zika antibodies with other flaviviruses, and nucleic acid and protein biomarkers for ZIKV are detectable at different stages of infection. Here, we describe a new optofluidic approach for the parallel detection of different molecular biomarkers using multimode interference (MMI) waveguides. We report differentiated, multiplex detection of both ZIKV biomarker types using multi-spot excitation at two visible wavelengths with over 98% fidelity by combining several analysis techniques.

Keywords: (130.3120) Integrated optics devices; (170.0170) Medical optics and biotechnology; (170.1610) Clinical applications; (230.7390) Waveguides, planar.