Genomic Characterization of Carbapenemase-Producing Klebsiella pneumoniae with Chromosomally Carried blaNDM-1

Antimicrob Agents Chemother. 2018 Nov 26;62(12):e01520-18. doi: 10.1128/AAC.01520-18. Print 2018 Dec.

Abstract

We report here Klebsiella pneumoniae strains carrying chromosomal blaNDM-1 in Thailand. The genomes of these two isolates include a 160-kbp insertion containing blaNDM-1, which is almost identical to that in the IncHI1B-like plasmid. Further analysis indicated that IS5-mediated intermolecular transposition and Tn3 transposase-mediated homologous recombination resulted in the integration of blaNDM-1 into the chromosome from an IncHI1B-like plasmid. The spread of this type of carbapenem-resistant Enterobacteriaceae may threaten public health and warrants further monitoring.

Keywords: IS5; Klebsiella pneumoniae; Tn3; beta-lactamases; blaNDM; carbapenemase-producing Enterobacteriaceae; carbapenems.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / therapeutic use
  • Carbapenems / therapeutic use
  • Chromosome Mapping
  • Chromosomes, Bacterial / chemistry*
  • DNA Transposable Elements
  • Gene Expression
  • Genome, Bacterial*
  • Homologous Recombination
  • Humans
  • Klebsiella Infections / drug therapy
  • Klebsiella Infections / epidemiology
  • Klebsiella Infections / microbiology
  • Klebsiella pneumoniae / drug effects
  • Klebsiella pneumoniae / enzymology
  • Klebsiella pneumoniae / genetics*
  • Klebsiella pneumoniae / isolation & purification
  • Mutagenesis, Insertional*
  • Plasmids / chemistry
  • Plasmids / metabolism*
  • Thailand / epidemiology
  • Transposases / genetics
  • Transposases / metabolism
  • beta-Lactamases / genetics*
  • beta-Lactamases / metabolism

Substances

  • Anti-Bacterial Agents
  • Carbapenems
  • DNA Transposable Elements
  • Transposases
  • beta-Lactamases
  • beta-lactamase NDM-1