Abstract
We report here Klebsiella pneumoniae strains carrying chromosomal blaNDM-1 in Thailand. The genomes of these two isolates include a 160-kbp insertion containing blaNDM-1, which is almost identical to that in the IncHI1B-like plasmid. Further analysis indicated that IS5-mediated intermolecular transposition and Tn3 transposase-mediated homologous recombination resulted in the integration of blaNDM-1 into the chromosome from an IncHI1B-like plasmid. The spread of this type of carbapenem-resistant Enterobacteriaceae may threaten public health and warrants further monitoring.
Keywords:
IS5; Klebsiella pneumoniae; Tn3; beta-lactamases; blaNDM; carbapenemase-producing Enterobacteriaceae; carbapenems.
Copyright © 2018 American Society for Microbiology.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anti-Bacterial Agents / therapeutic use
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Carbapenems / therapeutic use
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Chromosome Mapping
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Chromosomes, Bacterial / chemistry*
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DNA Transposable Elements
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Gene Expression
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Genome, Bacterial*
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Homologous Recombination
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Humans
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Klebsiella Infections / drug therapy
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Klebsiella Infections / epidemiology
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Klebsiella Infections / microbiology
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Klebsiella pneumoniae / drug effects
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Klebsiella pneumoniae / enzymology
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Klebsiella pneumoniae / genetics*
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Klebsiella pneumoniae / isolation & purification
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Mutagenesis, Insertional*
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Plasmids / chemistry
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Plasmids / metabolism*
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Thailand / epidemiology
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Transposases / genetics
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Transposases / metabolism
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beta-Lactamases / genetics*
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beta-Lactamases / metabolism
Substances
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Anti-Bacterial Agents
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Carbapenems
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DNA Transposable Elements
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Transposases
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beta-Lactamases
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beta-lactamase NDM-1