γ-oryzanol ameliorates the acute stress induced by behavioral anxiety testing in mice

Salina Akter; Hiroyuki Sasaki; Yuko Ikeda; Hiroki Miyakawa; Shigenobu Shibata

doi:10.1016/j.jphs.2018.06.003

γ-oryzanol ameliorates the acute stress induced by behavioral anxiety testing in mice

J Pharmacol Sci. 2018 Oct;138(2):155-159. doi: 10.1016/j.jphs.2018.06.003. Epub 2018 Jun 7.

Authors

Salina Akter¹, Hiroyuki Sasaki¹, Yuko Ikeda¹, Hiroki Miyakawa¹, Shigenobu Shibata²

Affiliations

¹ Laboratory of Physiology and Pharmacology, School of Advanced Science and Engineering, Waseda University, Tokyo, Japan.
² Laboratory of Physiology and Pharmacology, School of Advanced Science and Engineering, Waseda University, Tokyo, Japan. Electronic address: shibata@waseda.jp.

PMID: 30322802
DOI: 10.1016/j.jphs.2018.06.003

Abstract

We evaluated the anxiolytic effect of γ-oryzanol (GORZ) and elucidated the molecular mechanisms involved in its inhibition of behavioral test-induced anxiety. Behavioral tests were conducted on day 13, and mice were subjected to 30 min of acute restraint stress treatment (ARST) before sacrifice on day 16. In other group, behavioral tests were conducted on day 13 and 14 after ARST. 0.5% GORZ significantly weakened the effect of behavioral stress, but not the effect of strong ARST. GORZ downregulated ARST-induced cFos levels in the cerebral cortex. In conclusion, GORZ has potential ant-anxiety effect in the treatment of weak behavioral test-induced stress.

Keywords: Anxiety; Behavioral tests; cFos.

MeSH terms

Acute Disease
Animals
Anti-Anxiety Agents*
Anxiety / drug therapy*
Anxiety / etiology*
Anxiety / metabolism
Behavior, Animal / drug effects*
Cerebral Cortex / metabolism
Male
Mice, Inbred ICR
Phenylpropionates / administration & dosage*
Phenylpropionates / pharmacology*
Proto-Oncogene Proteins c-fos / metabolism
Psychological Tests
Stress, Psychological / complications*

Substances

Anti-Anxiety Agents
Phenylpropionates
Proto-Oncogene Proteins c-fos
gamma-oryzanol