Structural characterization and anti-proliferative activities of partially degraded polysaccharides from peach gum

Chaoyang Wei; Yu Zhang; Liang He; Junwen Cheng; Junhui Li; Wenyang Tao; Guizhu Mao; Hua Zhang; Robert J Linhardt; Xingqian Ye; Shiguo Chen

doi:10.1016/j.carbpol.2018.09.029

Structural characterization and anti-proliferative activities of partially degraded polysaccharides from peach gum

Carbohydr Polym. 2019 Jan 1:203:193-202. doi: 10.1016/j.carbpol.2018.09.029. Epub 2018 Sep 20.

Authors

Chaoyang Wei¹, Yu Zhang¹, Liang He², Junwen Cheng², Junhui Li¹, Wenyang Tao¹, Guizhu Mao¹, Hua Zhang¹, Robert J Linhardt³, Xingqian Ye⁴, Shiguo Chen⁵

Affiliations

¹ Laboratory of Food Processing and Engineering, Department of Food Science and Nutrition, College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China; Key Laboratory for Agro-Products Postharvest Handling of Ministry of Agriculture, Department of Food Science and Nutrition, College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China.
² Key Laboratory of Biological and Chemical Utilization of Zhejiang Forest Resources, Department of Forest Foods, Zhejiang Forestry Academy, Hangzhou 310023, China.
³ Departments of Chemistry and Chemical Biology, Chemical and Biological Engineering, Biology and Biomedical Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY, 12180, USA.
⁴ Laboratory of Food Processing and Engineering, Department of Food Science and Nutrition, College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China. Electronic address: psu@zju.edu.cn.
⁵ Laboratory of Food Processing and Engineering, Department of Food Science and Nutrition, College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China. Electronic address: chenshiguo210@163.com.

PMID: 30318204
DOI: 10.1016/j.carbpol.2018.09.029

Abstract

LP100R, LP10R and LP5R were isolated from peach gum by ultrafiltration. They were identified as AG II arabinogalactans composed of mannose, rhamnose, glucuronic acid, galactose, xylose and arabinose, which had a β-d-(1→6)-galactan backbone and were branched at O-3 and O-4. LP100R, LP10R and LP5R exist in a spherical conformation with the molecular weight of 8.50 × 10⁴ g/mol, 4.77 × 10⁴ g/mol and 2.40 × 10⁴ g/mol, respectively. The binding affinities of LP fractions to galectin-3 (Gal-3) were 0.77 μM for LP100R, 2.88 μM for LP10R and 5.15 μM for LP5R, respectively. Meanwhile, an anti-proliferative assay revealed that LP100R possessed higher anti-proliferative activity against HepG2 cells (IC₅₀, 4.5 mg/mL) and MCF-7 cells (IC₅₀, 0.43 mg/mL) than did LP10R and LP5R, which were in accordance with their binding affinities to galectin-3. Therefore, LP fractions (especially LP100R) might exert the anti-tumor activity by directly inhibiting the Gal-3 mediated proliferation of cancer cells.

Keywords: Anti-Proliferation activities; Gal-3 binding affinity; Molecular weight and chain conformation; Peach gum polysaccharides; Structural characterization.