Towards a Quantitative Understanding of Cell Identity

Abstract

Cells have traditionally been characterized using expression levels of a few proteins that are thought to specify phenotype. This requires a priori selection of proteins, which can introduce descriptor bias, and neglects the wealth of additional molecular information nested within each cell in a population, which often makes these sparse descriptors qualitative. Recently, more unbiased and quantitative cell characterization has been made possible by new high-throughput, information-dense experimental approaches and data-driven computational methods. This review discusses such quantitative descriptors in the context of three central concepts of cell identity: definition, creation, and stability. Collectively, these concepts are essential for constructing quantitative phenotypic landscapes, which will enhance our understanding of cell biology and facilitate cell engineering for research and clinical applications.

Keywords: cell phenotype; cellular decision making; computational modeling; high-throughput data analysis; network biology; phenotypic landscape.