Effects of protein S-nitrosylation on the glycogen metabolism in postmortem pork

Lili Zhang; Rui Liu; Yuping Cheng; Lujuan Xing; Guanghong Zhou; Wangang Zhang

doi:10.1016/j.foodchem.2018.08.103

Effects of protein S-nitrosylation on the glycogen metabolism in postmortem pork

Food Chem. 2019 Jan 30:272:613-618. doi: 10.1016/j.foodchem.2018.08.103. Epub 2018 Aug 23.

Authors

Lili Zhang¹, Rui Liu¹, Yuping Cheng¹, Lujuan Xing¹, Guanghong Zhou¹, Wangang Zhang²

Affiliations

¹ Key Laboratory of Meat Processing and Quality Control, Ministry of Education China, Jiangsu Collaborative Innovation Center of Meat Quality and Safety Control, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
² Key Laboratory of Meat Processing and Quality Control, Ministry of Education China, Jiangsu Collaborative Innovation Center of Meat Quality and Safety Control, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: wangang.zhang@njau.edu.cn.

PMID: 30309589
DOI: 10.1016/j.foodchem.2018.08.103

Abstract

The aim of this study was to investigate the effects of protein S-nitrosylation on the glycogen metabolism in postmortem pork. The pork samples were incubated with control (0.9% NaCl), nitric oxide synthase (NOS) inhibitor, or NO donor for 4 and 12 h at 4 °C. Results indicate that NOS inhibitor treatment led to significantly lower level of glycogen and higher lactate content at 24 h compared those of control and NO donor treatments (P < 0.05). The pH of NOS inhibitor treatment was significantly lower than other treatments, which indicates the fast glycolysis during postmortem aging (P < 0.05). In addition, the activities of glycolytic enzymes including GP, GAPDH and PK were significantly different among three treatments (P < 0.05) possibly due to the different modification of protein S-nitrosylation. These results suggest that NO could regulate the glycogen metabolism through modulating the activities of glycolytic enzymes by protein S-nitrosylation.

Keywords: Enzyme; Glycogen; Glycolysis; Lactate; Nitric oxide; S-nitrosylation.

MeSH terms

Animals
Glycogen / metabolism*
Glycogen Phosphorylase / metabolism
Glycolysis
Hydrogen-Ion Concentration
Muscle Proteins / chemistry
Muscle Proteins / metabolism*
Muscle, Skeletal / metabolism*
Nitric Oxide Donors / chemistry
Nitric Oxide Donors / metabolism
Nitric Oxide Synthase / antagonists & inhibitors
Protein Processing, Post-Translational
S-Nitrosothiols / analysis*
Swine

Substances

Muscle Proteins
Nitric Oxide Donors
S-Nitrosothiols
Glycogen
Nitric Oxide Synthase
Glycogen Phosphorylase