A Hemicyanine-Embedded Diphenylselenide-Containing Probe "HemiSe" in which SePh2 Stays Reduced for Selective Detection of Superoxide in Living Cells

Chem Asian J. 2018 Dec 18;13(24):3895-3902. doi: 10.1002/asia.201801339. Epub 2018 Nov 14.

Abstract

A simple one-step synthesis of fluorescent probe HemiSe has been developed for the detection of superoxide (O2 .- ). The probe undergoes reaction specifically with O2 .- when in the presence of other competitive ROS/RNS/metal ions. The diphenylselenide was incorporated to completely quench the fluorescence of the hemicyanine unit through the action of a photoinduced electron transfer (PET) photomechanism. However, after the addition of O2 .- , the latent fluorophore regains its fluorescence owing to the reaction at the C=C bond of the hemicyanine with O2 .- through nucleophilic attack; the increase in blue emission is due to a reaction of the double bond within HemiSe followed by an increase in fluorescence quantum yield (Φ) up to 0.45; the limit of detection (LOD) is 11.9 nm. A time-dependent study shows that HemiSe can detect superoxide within 13 min with high sensitivity, high selectivity, over a wide pH range, and through confirmation with a xanthine/xanthine oxidase biochemical assay (λem =439 nm). A study in the RAW 264.7 macrophage living cells also shows that HemiSe is not toxic, cell permeable (experimental log P=2.11); confocal imaging results show that HemiSe can detect O2 .- in endogenous and exogeneous systems.

Keywords: fluorescence; live cell imaging; phenylselenides; radicals; reactive oxygen species.

MeSH terms

  • Animals
  • Carbocyanines / chemistry*
  • Fluorescent Dyes / chemistry*
  • Mice
  • Microscopy, Confocal / methods
  • Optical Imaging / methods
  • Organoselenium Compounds / chemistry*
  • Oxidation-Reduction
  • RAW 264.7 Cells
  • Spectrometry, Fluorescence / methods
  • Superoxides / analysis*

Substances

  • Carbocyanines
  • Fluorescent Dyes
  • Organoselenium Compounds
  • tetramethylene hemicyanine
  • Superoxides