Evaluation of pulmonary artery stenosis in congenital heart disease patients using functional diagnostic parameters: An in vitro study

Abstract

Congenital pulmonary artery (PA) stenosis is often associated with abnormal PA hemodynamics including increased pressure drop (Δp) and reduced asymmetric flow (Q), which may result in right ventricular dysfunction. We propose functional diagnostic parameters, pressure drop coefficient (CDP), energy loss (E_loss), and normalized energy loss (E¯_loss) to characterize pulmonary hemodynamics, and evaluate their efficacy in delineating stenosis severity using in vitro experiments. Subject-specific test sections including the main PA (MPA) bifurcating into left and right PAs (LPA, RPA) with a discrete LPA stenosis were manufactured from cross-sectional imaging and 3D printing. Three clinically-relevant stenosis severities, 90% area stenosis (AS), 80% AS, and 70% AS, were evaluated at different cardiac outputs (COs). A benchtop flow loop simulating pulmonary hemodynamics was used to measure Q and Δp within the test sections. The experimental Δp-Q characteristics along with clinical data were used to obtain pathophysiologic conditions and compute the diagnostic parameters. The pathophysiologic Q_LPA decreased as the stenosis severity increased at a fixed CO. CDP_LPA, E_loss,LPA (absolute), and E¯_loss,LPA (absolute) increased with an increase in LPA stenosis severity at a fixed CO. Importantly, CDP_LPA and E¯_loss,LPA had reduced variability with CO, and distinct values for each LPA stenosis severity. Under variable CO, a) CDP_LPA values were 14.5-21.0 (70% AS), 60.7- 2.2 (80% AS), ≥ 261.6 (90% AS), and b) E¯_loss,LPA values (in mJ per Q_LPA) were -501.9 to -1023.8 (70% AS), -1247.6 to -1773.0 (80% AS), -1934.5 (90% AS). Hence, CDP_LPA and E¯_loss,LPA are expected to assess the true functional severity of PA stenosis.

Keywords: Congenital heart disease; Functional diagnostic parameters; In vitro experiments; Pulmonary artery stenosis; Subject-specific.