This work explores the use of multispectral imaging (MSI) techniques applied to the investigation of Late Antique (c. 250-800 AD) textiles found in Egypt. Although the use of these techniques is well-established in the study of polychrome surfaces, they have only been sparingly and often unsystematically applied to the investigation of textiles. The aim of this work is therefore to bridge this gap by showing how this non-invasive, relatively inexpensive and portable methodology can be used to map the photoluminescence and reflective characteristics of textiles under different wavelengths of light, and to provide qualitative and holistic insights into the chemical nature of the materials that compose them. Standardised acquisition and post-processing methods were applied to produce visible-reflected (VIS), ultraviolet-induced visible luminescence (UVL), infrared-reflected (IRR), infrared-reflected false colour (IRRFC), ultraviolet-reflected (UVR) and ultraviolet-reflected false colour (UVRFC) images that provided preliminary indications of the colourants used and their spatial distribution. This proved to be an important aid in planning more targeted and effective sampling strategies and facilitated comparisons between objects. Visible-induced visible luminescence (VIVL) and multiband-reflected (MBR) imaging were also explored for the first time with application to textiles, demonstrating their potential in mapping red and blue colourants respectively. The physical properties observed from all of these images were then related to the more detailed information provided by complementary non-invasive techniques, such as fibre optic reflectance spectroscopy (FORS), and micro-invasive approaches, such as high-performance liquid chromatography mass spectrometry (HPLC-MS). Guidelines towards the interpretation of complex MSI images and a discussion of the potential and limitations of relating multispectral data to chemical properties are presented. An important result of this work is the delineation of a protocol, which combines optical microscopy (OM), MSI, FORS and HPLC-MS and shows a high degree of potential, not only for the investigation of Late Antique textiles but for textiles in museum and historic collections generally.