Noncovalent spin-labeling of RNA: the aptamer approach

Chem Commun (Camb). 2018 Oct 16;54(83):11749-11752. doi: 10.1039/c8cc05597a.

Abstract

In the first example of site-directed spin-labeling of unmodified RNA, a pyrrolidine-nitroxide derivative of tetramethylrosamine (TMR) was shown to bind with high affinity to the malachite green (MG) aptamer, as determined by continuous-wave (CW) electron paramagnetic resonance (EPR), pulsed electron-electron double resonance (PELDOR) and fluorescence spectroscopies.

MeSH terms

  • Aptamers, Nucleotide / chemistry*
  • Binding Sites
  • Electron Spin Resonance Spectroscopy
  • Heterocyclic Compounds, 3-Ring / chemistry
  • Models, Molecular
  • Pyrrolidines / chemistry
  • RNA / chemistry*
  • Rhodamines
  • Rosaniline Dyes / analysis
  • Spectrometry, Fluorescence
  • Spin Labels* / chemical synthesis

Substances

  • Aptamers, Nucleotide
  • Heterocyclic Compounds, 3-Ring
  • Pyrrolidines
  • Rhodamines
  • Rosaniline Dyes
  • Spin Labels
  • tetramethylrosamine
  • malachite green
  • RNA
  • pyrrolidine