Abstract
In the first example of site-directed spin-labeling of unmodified RNA, a pyrrolidine-nitroxide derivative of tetramethylrosamine (TMR) was shown to bind with high affinity to the malachite green (MG) aptamer, as determined by continuous-wave (CW) electron paramagnetic resonance (EPR), pulsed electron-electron double resonance (PELDOR) and fluorescence spectroscopies.
MeSH terms
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Aptamers, Nucleotide / chemistry*
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Binding Sites
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Electron Spin Resonance Spectroscopy
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Heterocyclic Compounds, 3-Ring / chemistry
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Models, Molecular
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Pyrrolidines / chemistry
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RNA / chemistry*
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Rhodamines
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Rosaniline Dyes / analysis
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Spectrometry, Fluorescence
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Spin Labels* / chemical synthesis
Substances
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Aptamers, Nucleotide
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Heterocyclic Compounds, 3-Ring
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Pyrrolidines
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Rhodamines
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Rosaniline Dyes
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Spin Labels
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tetramethylrosamine
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malachite green
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RNA
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pyrrolidine