Surgical repair of abdominal wall defect with biomimetic nano/microfibrous hybrid scaffold

Abstract

It is universal to repair abdominal wall defects with prosthetic materials in abdominal surgery worldwide, which are associated with high complications and organ damage. At present, the composite nanofibers composed of natural and synthetic polymers as the new type of nano structure scaffold have attracted considerable attention in the field of tissue engineering. In this study we examined the feasibility of using electrospun silk fibroin (SF)/poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) hybrid scaffolds for repairing of abdominal wall defects. Both in vivo and in vitro characterization were evaluated to access efficacy of the nanofiber for tissue regeneration. Our results showed that the electrospun SF/PHBV nanofiber scaffolds could stimulate the expression of TGF-β1 and Collagen I in fibroblasts in vitro and then promote granulation and connective tissue depositions, but not result in a strong foreign body reaction in vivo. Moreover, we conjectured the potential molecular biological mechanism of SF/PHBV hybrid scaffolds in the process of tissue regeneration. Thus, the SF/PHBV hybrid nanofiber scaffolds have high efficiency and biocompatibility to repair abdominal wall defects.

Keywords: Abdominal wall defects; Hybrid scaffold; Poly (3-hydroxybutyrate-co-3-hydroxyvalerate); Silk fibroin; Tissue regeneration.