In the frame of the presented research, highly-porous structures made of hyaluronic acid modified with bioactive compounds were prepared. The method of microbiological testing of hygroscopic materials has been elaborated by verification of the JIS L 1902:2002 and ASTM E2149-13a test methods. The research has shown that the method developed in accordance with ASTM E2149-13a is suitable for testing the activity of hyaluronic acid samples against bacteria. E. coli and S. aureus. In the case of E. coli it is preferred to use as a medium the phosphoric buffer and for S. aureus NaCl solution from NB. By analysing the results of the antibacterial properties, it is important to note that the introduction of a small amount of zinc and zinc oxide in the matrix from the hyaluronic acid (in the amount of 3% by weight of the polymer) makes it possible to obtain a material with a strong activity against the bacterial strains. It enables to use this type of material as a treatment for hard-to-treat, infected wounds. On the other hand, using a relatively small dose of the cephalosporin antibiotic did not result in high levels of activity against the bacteria Gram "+" and Gram "-".
Keywords: Anti-bacterial; Hyaluronic acid; Methodology; Porous structures.
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