A yellow-pigmented, Gram-staining-negative, aerobic, non-motile and rod-shaped bacterium, designated strain F30T, was isolated from fresh water of a diseased farmed Murray cod with a profound ulceration pond in Zhejiang province, PR China. Growth was observed at NaCl concentrations of 0.5-3.5 % (w/v) (optimum, 1.5-2.0 %), temperatures of 10-35 °C (optimum, 28 °C) and pH 5.0-9.0 (optimum, 6.5). Phylogenetic analysis based on 16S rRNA gene sequences indicated that F30T represented a member of the genus Chryseobacterium, showing the highest similarity to Chryseobacterium jejuense DSM 19299T (99.0 %) and Chryseobacterium nakagawai NCTC 13529T (99.0 %), and less than 98.7 % similarity to other species of the genus Chryseobacterium with validly published names. The average nucleotide identity and in silico DNA-DNA hybridization values between F30T and the reference strains were 78.4-90.5 % and 2.6-42.5 %, respectively. The results of chemotaxonomic analysis indicated that the fatty acids, as well as the polar lipid profiles of F30T were similar to those of species of the genus Chryseobacterium, and the sole respiratory quinone was MK-6. On the basis of its phylogenetic, chemotaxonomic and phenotypic data, strain F30T represents a novel species, for which the name Chryseobacteriumaurantiacum sp. nov. is proposed. The type strain is F30T (=KCTC 62135T=MCCC 1K03457T).
Keywords: Chryseobacterium aurantiacum sp. nov.; Murray cod (Maccullochella peelii peelii); taxonomy.