Phosphoproteomics in Microbiology: Protocols for Studying Streptomyces coelicolor Differentiation

Methods Mol Biol. 2018:1841:249-260. doi: 10.1007/978-1-4939-8695-8_17.

Abstract

The extension and biological role of Ser/Thr/Tyr phosphorylation in prokaryotes have been only scarcely studied. In this chapter, we describe the state of the art of microbial phosphoproteomics, focusing on protocols used for studying the phosphoproteome of Streptomyces coelicolor, one of the bacteria encoding the largest number of eukaryote-like Ser/Thr/Tyr kinases.

Keywords: Differentiation; LC-MS/MS; Phosphoproteomics; Sporulation; Streptomyces.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / isolation & purification
  • Bacterial Proteins / metabolism*
  • Chromatography, Liquid
  • Phosphoproteins / isolation & purification
  • Phosphoproteins / metabolism*
  • Proteome*
  • Proteomics* / methods
  • Reproducibility of Results
  • Streptomyces coelicolor / metabolism*
  • Tandem Mass Spectrometry

Substances

  • Bacterial Proteins
  • Phosphoproteins
  • Proteome