Double-pulse laser illumination method for measuring fast cerebral blood flow velocities in the deep brain using a fiber-bundle-based endomicroscopy system

Minkyung Kim; Jinki Hong; Hyun-Joon Shin

doi:10.1364/BOE.9.002699

Double-pulse laser illumination method for measuring fast cerebral blood flow velocities in the deep brain using a fiber-bundle-based endomicroscopy system

Biomed Opt Express. 2018 May 21;9(6):2699-2715. doi: 10.1364/BOE.9.002699. eCollection 2018 Jun 1.

Authors

Minkyung Kim^{1

2}, Jinki Hong^{1

2}, Hyun-Joon Shin^{1

2}

Affiliations

¹ Center for Bionics, Biomedical Research Institute, Korea Institute of Science and Technology, Seoul 02792, South Korea.
² Biomedical Engineering, KIST School, UST, Korea University of Science and Technology, Seoul 02792, South Korea.

Abstract

We present a new fiber-bundle-based endomicroscopy system to measure the fast cerebral blood flow (CBF) velocity in blood vessels located between the surface and the deep brain of living animals. The CBF velocity is obtained by measuring the displacement of the partially overlapped red blood cell images directly, using double-pulse 532-nm laser illumination. The proposed method could measure CBF in blood vessels with diameters ranging from 4 μm to 42 μm and could measure CBF velocities up to 3.2 μm/ms for different vessel diameters at a depth of 2.1 mm from the brain surface in a living mouse.

Keywords: (110.2350) Fiber optics imaging; (130.0130) Integrated optics; (170.1470) Blood or tissue constituent monitoring; (170.2150) Endoscopic imaging; (170.2520) Fluorescence microscopy.