Two stochastic sensors based on the modification of graphite paste with the complexes formed by phthalocyanine (PhCN) with Ni and Cu were designed and used for molecular recognition of IL-8, IL-10, IL-12, and IL-15. The four interleukins were recognized according to their signatures-called toff (qualitative parameter) from the diagrams obtained after measurements. The limit of determination for IL-8 was 1 × 10-4μg mL-1 when both stochastic sensors were used; for IL-10, the determination limit was 4.5 × 10-4μg mL-1 for the Ni complex-based sensor, and 4.5 × 10-7μg mL-1 for the Cu complex-based sensor, respectively; for IL-12, the determination limit was 5 × 10-4μg mL-1 for the Ni complex-based sensor, and 5 × 10-7μg mL-1 for the Cu complex-based sensor, respectively; while for IL-15, the determination limit was 5 × 10-5μg/mL for the Ni complex-based sensor, and 5 × 10-5μg/mL for the Cu complex-based sensor, respectively. The stochastic method used was validated using the following biological fluids: nasal lavage, saliva, serum, and whole blood. Graphical abstract ᅟ.
Keywords: Biological fluids; Interleukin; Stochastic sensors.