Surveillance, isolation and complete genome sequence of bovine parainfluenza virus type 3 in Egyptian cattle

Nader M Sobhy; Sunil K Mor; Iman M Bastawecy; Hiam M Fakhry; Christiana R B Youssef; Sagar M Goyal

doi:10.1016/j.ijvsm.2017.02.004

Surveillance, isolation and complete genome sequence of bovine parainfluenza virus type 3 in Egyptian cattle

Int J Vet Sci Med. 2017 Apr 21;5(1):8-13. doi: 10.1016/j.ijvsm.2017.02.004. eCollection 2017 Jun.

Authors

Nader M Sobhy¹, Sunil K Mor², Iman M Bastawecy³, Hiam M Fakhry⁴, Christiana R B Youssef⁵, Sagar M Goyal²

Affiliations

¹ Department of Animal Medicine, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Sharkia 44511, Egypt.
² Department of Veterinary Population Medicine, University of Minnesota, St. Paul, MN 55108, USA.
³ Department of Virology, Animal Health Research Institute, Dokki 12618, Giza, Egypt.
⁴ Veterinary Serum and Vaccine Research Institute, East of Cairo 131, Egypt.
⁵ Department of Microbiology, Faculty of Pharmacy, Zagazig University, Zagazig, Sharkia 44519, Egypt.

Abstract

Parainfluenza virus type 3 (PIV-3) can infect a wide variety of mammals including humans, domestic animals, and wild animals. In the present study, bovine parainfluenza virus type 3 (BPIV-3) was isolated from nasal swabs of Egyptian cattle presenting with clinical signs of mild pneumonia. The virus was isolated in Madin-Darby bovine kidney (MDBK) cells and confirmed by reverse transcription-polymerase chain reaction (RT-PCR). The complete genome of Egyptian BPIV-3 strain was sequenced by using next generation (Illumina) sequencing. The new isolate classified with genotype A of BPIV-3 and was closely related to the Chinese NM09 strain (JQ063064). Subsequently in 2015-16, a molecular surveillance study was undertaken by collecting and testing samples from cattle and buffaloes with respiratory tract infections. The survey revealed a higher rate of BPIV-3 infection in cattle than in buffaloes. The infection was inversely proportional to the age of the animals and to warm weather. This report should form a basis for further molecular studies on animal viruses in Egypt.

Keywords: BPIV-3; Cattle; Egypt; Parainfluenza; Phylogenetic analysis.