Modulation of age related protein expression changes by gelam honey in cardiac mitochondrial rats

Siti Maisarah Hasenan; Saiful Anuar Karsani; Zakiah Jubri

doi:10.1016/j.exger.2018.09.001

Modulation of age related protein expression changes by gelam honey in cardiac mitochondrial rats

Exp Gerontol. 2018 Nov:113:1-9. doi: 10.1016/j.exger.2018.09.001. Epub 2018 Sep 21.

Authors

Siti Maisarah Hasenan¹, Saiful Anuar Karsani², Zakiah Jubri³

Affiliations

¹ Department of Biochemistry, Medical Centre of National University of Malaysia, Jalan Yaacob Latif, Bandar Tun Razak, 56000, Cheras, Kuala Lumpur, Malaysia.
² Institute of Biological Sciences, Faculty of Science, University of Malaya and University of Malaya Centre for Proteomics Research (UMCPR), Kuala Lumpur, Malaysia. Electronic address: saiful72@um.edu.my.
³ Department of Biochemistry, Medical Centre of National University of Malaysia, Jalan Yaacob Latif, Bandar Tun Razak, 56000, Cheras, Kuala Lumpur, Malaysia. Electronic address: zakiah.jubri@ppukm.ukm.edu.my.

PMID: 30248357
DOI: 10.1016/j.exger.2018.09.001

Abstract

Aging is characterized by progressive decline in biochemical and physiological functions. According to the free radical theory of aging, aging results from oxidative damage due to the accumulation of excess reactive oxygen species (ROS). Mitochondria are the main source of ROS production and are also the main target for ROS. Therefore, a diet high in antioxidant such as honey is potentially able to protect the body from ROS and oxidative damage. Gelam honey is higher in flavonoid content and phenolic compounds compared to other local honey. This study was conducted to determine the effects of gelam honey on age related protein expression changes in cardiac mitochondrial rat. A total of 24 Sprague-Dawley male rats were divided into two groups: the young group (2 months old), and aged group (19 months old). Each group were then subdivided into two groups: control group (force-fed with distilled water), and treatment group (force-fed with gelam honey, 2.5 g/kg), and were treated for 8 months. Comparative proteomic analysis of mitochondria from cardiac tissue was then performed by high performance mass spectrometry (Q-TOF LCMS/MS) followed by validation of selected proteins by Western blotting. Proteins were identified using Spectrum Mill software and were subjected to stringent statistical analysis. A total of 286 proteins were identified in the young control group (YC) and 241 proteins were identified in the young gelam group (YG). In the aged group, a total of 243 proteins were identified in control group (OC), and 271 proteins in gelam group (OG). Comparative proteome profiling identified 69 proteins with different abundance (p < 0.05) in OC when compared to YC, and also in YG when compared to YC. On the other hand, 55 proteins were found to be different in abundance when comparing OG with OC. In the aged group, gelam honey supplementation affected the relative abundance of 52 proteins with most of these proteins showing a decrease in the control group. Bioinformatics analysis showed that the majority of the affected proteins were involved in the respiratory chain (OXPHOS) which play an important role in maintaining mitochondrial function.

Keywords: Aging; Antioxidant; Gelam honey; Mitochondria; Proteomic.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aging*
Animals
Antioxidants / chemistry
Dietary Supplements
Flavonoids / chemistry
Honey*
Male
Mitochondria, Heart / physiology*
Oxidation-Reduction
Oxidative Phosphorylation*
Oxidative Stress*
Rats
Rats, Sprague-Dawley
Reactive Oxygen Species / metabolism*

Substances

Antioxidants
Flavonoids
Reactive Oxygen Species