In recent years, incidences of invasive fungal infections have greatly increased, especially in immunosuppressed patients, but most today's antifungal drugs are not completely effective due to the development of drug resistance, as well as potential toxicity and adverse effects. Consequently, it is imperative to search for novel antifungal agents to combat fungal infections. This review will discuss the advances in the traditional antifungal therapy, and present an overview of novel strategies for the treatment of fungal infections. The papers presented here highlight new targets that could be exploited for development of new antifungal agents.