Inhibition of Leishmania amazonensis arginase by fucogalactan isolated from Agrocybe aegerita mushroom

Renan Akio Motoshima; Tainara da F Rosa; Léia da C Mendes; Estefânia Viana da Silva; Sthefany R F Viana; Bruno Sérgio do Amaral; Dulce H F de Souza; Luciano M Lião; Maria de Lourdes Corradi da Silva; Lorena R F de Sousa; Elaine R Carbonero

doi:10.1016/j.carbpol.2018.08.109

Inhibition of Leishmania amazonensis arginase by fucogalactan isolated from Agrocybe aegerita mushroom

Carbohydr Polym. 2018 Dec 1:201:532-538. doi: 10.1016/j.carbpol.2018.08.109. Epub 2018 Aug 27.

Affiliations

¹ Unidade Acadêmica Especial de Química, Universidade Federal de Goiás, Regional Catalão, 75704-020, Catalão, GO, Brazil.
² Unidade Acadêmica Especial de Química, Universidade Federal de Goiás, Regional Catalão, 75704-020, Catalão, GO, Brazil; Laboratório de Ressonância Magnética Nuclear, Instituto de Química, Universidade Federal de Goiás, Campus Samambaia, 74001-970, Goiânia, GO, Brazil.
³ Departamento de Engenharia Rural, Faculdade de Ciências Agronômicas, Universidade Estadual Paulista "Júlio de Mesquita Filho", 18610-307, Botucatu, SP, Brazil.
⁴ Departamento de Química, Universidade Federal de São Carlos, Rodovia Washington Luís, Km 235, 13565-905, São Carlos, SP, Brazil.
⁵ Laboratório de Ressonância Magnética Nuclear, Instituto de Química, Universidade Federal de Goiás, Campus Samambaia, 74001-970, Goiânia, GO, Brazil.
⁶ Departamento de Química e Bioquímica, Faculdade de Ciências e Tecnologia, Universidade Estadual Paulista "Júlio de Mesquita Filho", 19060-900, Presidente Prudente, SP, Brazil.
⁷ Unidade Acadêmica Especial de Química, Universidade Federal de Goiás, Regional Catalão, 75704-020, Catalão, GO, Brazil. Electronic address: elaine_carbonero@ufg.br.

PMID: 30241850
DOI: 10.1016/j.carbpol.2018.08.109

Abstract

The inhibition of arginase from Leishmania spp. is considered a promising approach to the leishmaniasis treatment. In this study, the potential of a fucogalactan isolated from the medicinal mushroom Agrocybe aegerita was evaluated against arginase (ARG) from Leishmania amazonensis. The polysaccharide was obtained via aqueous extraction, and purified by freeze thawing and precipitation with Fehling solution. Its chemical structure was established by monosaccharide composition, methylation analysis, partial acid hydrolysis, and NMR spectroscopy. The data indicated that it is a fucogalactan (FG-Aa; M_w = 13.8 kDa), having a (1→6)-linked α-D-Galp main-chain partially substituted in O-2 by non-reducing end-units of α-L-Fucp. FG-Aa showed significant inhibitory activity on ARG with IC₅₀potency of 5.82 ± 0.57 μM. The mechanism of ARG inhibition by the heterogalactan was the competitive type, with K_iof 1.54 ± 0.15 μM. This is the first report of an inhibitory activity of arginase from L. amazonensis by biopolymers, which encourages us to investigate further polysaccharides as a new class of ARG inhibitors.

Keywords: Agrocybe aegerita; Arginase; Chemical structure; Competitive inhibitor; Fucogalactan; Leishmania amazonensis.

MeSH terms

Agrocybe / chemistry*
Arginase / antagonists & inhibitors*
Arginase / chemistry
Enzyme Inhibitors / chemistry*
Fungal Polysaccharides / chemistry*
Galactans / chemistry*
Leishmania / enzymology*
Protozoan Proteins / antagonists & inhibitors*
Protozoan Proteins / chemistry

Substances

Enzyme Inhibitors
Fungal Polysaccharides
Galactans
Protozoan Proteins
fucogalactan
Arginase