Structure characterization and hypoglycemic activity of an arabinogalactan from Phyllostachys heterocycla bamboo shoot shell

Huanyan Liu; Pengfei He; Liang He; Qin Li; Junwen Cheng; Yanbin Wang; Guang Yang; Bo Yang

doi:10.1016/j.carbpol.2018.08.024

Structure characterization and hypoglycemic activity of an arabinogalactan from Phyllostachys heterocycla bamboo shoot shell

Carbohydr Polym. 2018 Dec 1:201:189-200. doi: 10.1016/j.carbpol.2018.08.024. Epub 2018 Aug 13.

Authors

Huanyan Liu¹, Pengfei He², Liang He³, Qin Li⁴, Junwen Cheng⁵, Yanbin Wang⁵, Guang Yang⁶, Bo Yang⁶

Affiliations

¹ Bamboo Shoots Engineering Research Center of the State Forestry Bureau, Department of Forest Foods, Zhejiang Academy of Forestry, Hangzhou 310023, China; School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China.
² Department of Processing, Marine Fisheries Research Institute of Zhejiang, Zhoushan 316021, China.
³ Bamboo Shoots Engineering Research Center of the State Forestry Bureau, Department of Forest Foods, Zhejiang Academy of Forestry, Hangzhou 310023, China. Electronic address: kite006@126.com.
⁴ Bamboo Shoots Engineering Research Center of the State Forestry Bureau, Department of Forest Foods, Zhejiang Academy of Forestry, Hangzhou 310023, China. Electronic address: 18258174992@139.com.
⁵ Bamboo Shoots Engineering Research Center of the State Forestry Bureau, Department of Forest Foods, Zhejiang Academy of Forestry, Hangzhou 310023, China.
⁶ School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China.

PMID: 30241810
DOI: 10.1016/j.carbpol.2018.08.024

Abstract

An arabinogalactan (PBSS2) was fractionated from the bamboo shoot shell of Phyllostachys heterocycla. Structural analysis indicated that PBSS2 was mainly composed of galactose, arabinose, xylose and galacturonic acid in a ratio of 7.8:6.3:1.4:1.0. It was a 1,3- linked β-d-galactan having 61.1% degree of branching at the O-6 positions. The three branches consisting of 1,4-linked β-d-Xylp terminated with β-d-Galp, 1,5-linked α-L-Araf inserted with 1,4-α-6-O-Me-d-GalpA and 1,3,5-linked α-L-Araf terminated by α-L-Araf. Furthermore, its chain conformation on the values of weight-average molecular weight (M_w), the radius of gyration (R_g) and intrinsic viscosity ([η]) were found as following: 7.36 × 10⁴ g/mol, 12.8 nm and 17.7 mL/g, which was evidenced by AFM. The structure exponent of α (0.38) and d_f (2.17) revealed it existed as a sphere-like chain in NaNO₃ aqueous solution. In vitro Caco-2 cells assay showed that PBSS2 presented positive effect on the inhibition of glucose absorption in time-dependent manner at relatively high concentration.

Keywords: Arabinogalactan; Hypoglycemic activity; Phyllostachys heterocycle bamboo shoot shell; Structural characterization.