Prolonged DNA hydrolysis in water: A study on DNA stability

Paolo Fattorini; Giorgio Marrubini; Serena Bonin; Barbara Bertoglio; Pierangela Grignani; Elisa Recchia; Paola Pitacco; Francesca Procopio; Carolina Cantoni; Irena Zupanič Pajnič; Solange Sorçaburu-Cigliero; Carlo Previdere

doi:10.1016/j.dib.2018.08.120

Prolonged DNA hydrolysis in water: A study on DNA stability

Data Brief. 2018 Aug 30:20:1237-1243. doi: 10.1016/j.dib.2018.08.120. eCollection 2018 Oct.

Affiliations

¹ Department of Medicine, Surgery and Health, University of Trieste, Italy.
² Department of Drug Sciences, University of Pavia, Italy.
³ Department of Public Health, Experimental and Forensic Medicine, Section of Legal Medicine and Forensic Sciences, University of Pavia, Italy.
⁴ School of Biological and Chemical Sciences, Queen Mary University of London, United Kingdom.
⁵ CNR-ISMAR, Trieste, Italy.
⁶ Institute of Forensic Medicine, Faculty of Medicine, University of Ljubljana, Slovenia.

Abstract

This work provides a protocol for the in vitro production of damaged DNA samples. In particular, heat-mediated hydrolysis of the samples at 70 °C in ultrapure water was performed in 1.7 mL Eppendorf tubes sealed by Parafilm for 0-36 h. The chemical/physical features of the resulting samples are described. After normalization of the qPCR data, these were compared with those obtained from samples treated for 0-10 h in a previous study.

Keywords: DNA hydrolysis; DNA quantification; Degraded samples; qPCR.