Transcriptomic data on the role of PEST-domain-enriched tyrosine phosphatase in the regulation of antigen-mediated activation and antiallergic action of glucocorticoids in mast cells

George K Ainooson; Victor Gourain; Michael Stassen; Andrew C B Cato

doi:10.1016/j.dib.2018.08.188

Transcriptomic data on the role of PEST-domain-enriched tyrosine phosphatase in the regulation of antigen-mediated activation and antiallergic action of glucocorticoids in mast cells

Data Brief. 2018 Sep 5:20:1177-1183. doi: 10.1016/j.dib.2018.08.188. eCollection 2018 Oct.

Authors

George K Ainooson¹, Victor Gourain¹, Michael Stassen², Andrew C B Cato¹

Affiliations

¹ Karlsruhe Institute of Technology, Institute of Toxicology and Genetics, Hermann-von-Helmholtz-Platz-1, 76344 Eggenstein-Leopoldshafen, Germany.
² Institute for Immunology, University Medical Center, Johannes Gutenberg University, Langenbeckstr. 1, 55131 Mainz, Germany.

Abstract

Protein tyrosine phosphatases and glucocorticoids are known to regulate allergic and antiallergic action in activated mast cells. Here we provide RNA sequencing and quantitative real-time PCR data from bone marrow derived mast cells, for wild-type and PEST-domain-enriched tyrosine phosphatase (PEP) null mice, activated by immunoglobulin E sensitization and dinitrophenol treatment, and additionally treated with the glucocorticoid dexamethasone. The transcriptomics experiment was performed in duplicate with a total of 16 samples (GSE108972).

Keywords: Allergy; BMMCs, Bone marrow derived mast cells; COX-2, Cyclooxygenase 2; CSF2, Colony stimulating factor 2; DAVID, Database for Annotation, Visualization and Integrated Discovery; DEX, Dexamethasone; DNP, Dinitrophenol; Gene expression; IL 13, Interleukin 13; IMDM, Iscove׳s Modified Dulbecco׳s Medium; IgE, Immunoglobulin E; KEGG, Kyoto Encyclopedia of Genes and Genomes; PCA, Principal Component Analysis; PEP, PEST-domain-enriched tyrosine phosphatase; PTGDS, Prostaglandin D2 synthase - lipocalin type; Phosphatase; Quantitative real-time PCR; RNA sequencing; RNA-seq, RNA-sequencing; SBS, Sequencing by Synthesis; TNFα, Tumor necrosis factor alpha; qRT-PCR, Quantitative real-time polymerase chain reaction.