An in vitro and in silico study on the antioxidant and cell culture-based study on the chemoprotective activities of fish muscle protein hydrolysates obtained from European seabass and gilthead seabream

Abstract

European seabass (Dicentrarchus labrax, Linnaeus, 1758) (L) and gilthead seabream (Sparus aurata, Linnaeus, 1758) (C) muscles were hydrolysated by Alcalase (L_alc, C_alc) and Chymotrypsin (L_ch, C_ch) then hydrolysates were examined and their peptide profiles obtained. A total of 765, 794, 132 and 232 peptides were identified in C_alc, L_alc, C_ch and L_ch, respectively. Although, L_ch and C_ch were expected to have more antioxidant capacity because of their peptide profiles, Alcalase hydrolysates observed in vitro, were slightly higher (TEAC assay for C_alc: 848.11 ± 60.78 μmol TE/g protein). Maximum inhibition of oxidative stress was determined for L_alc (12.8% ± 4.5%) in MDCK1 cell lines. Highest proliferative capacity observed for C_alc (147.0% ± 3.1%) at MTT assay in MDCK1 cell culture. L_ch showed the highest chemopreventive effect with a 40-60% decrease for human colon adenocarcinoma cell line HT-29. This research points out the importance of aquatic sources as raw materials for peptide researches.

Keywords: Antioxidant; Chemopreventive; Enzymatic; Hydrolysate; Proliferative; Seabass; Seabream.