Transcriptome analysis and histopathology of black carp (Mylopharyngodon piceus) spleen infected by Aeromonas hydrophila

Xueshu Zhang; Yubang Shen; Xiaoyan Xu; Meng Zhang; Yulin Bai; Yiheng Miao; Yuan Fang; Jiahua Zhang; Rongquan Wang; Jiale Li

doi:10.1016/j.fsi.2018.09.047

Transcriptome analysis and histopathology of black carp (Mylopharyngodon piceus) spleen infected by Aeromonas hydrophila

Fish Shellfish Immunol. 2018 Dec:83:330-340. doi: 10.1016/j.fsi.2018.09.047. Epub 2018 Sep 15.

Authors

Xueshu Zhang¹, Yubang Shen², Xiaoyan Xu², Meng Zhang¹, Yulin Bai¹, Yiheng Miao¹, Yuan Fang¹, Jiahua Zhang¹, Rongquan Wang³, Jiale Li⁴

Affiliations

¹ Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Ocean University, Shanghai, China.
² Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Ocean University, Shanghai, China; Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, China.
³ Key Laboratory of Conventional Freshwater Fish Breeding and Health Culture Technology Germplasm Resources, Suzhou Shenhang Eco-technology Development Limited Company, Suzhou, PR China.
⁴ Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Ocean University, Shanghai, China; Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, China. Electronic address: jlli2009@126.com.

PMID: 30227254
DOI: 10.1016/j.fsi.2018.09.047

Abstract

Aeromonas hydrophila causes serious economic losses to the black carp (Mylopharyngodon piceus) industry. In this study, we analyzed the spleen of disease-resistant and susceptible black carp by RNA-seq. Overall, a total of 5243 terms were enriched in the gene ontology (GO) analysis, and 323 related pathways were found in the Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis. A total of 1935 differentially expressed genes were found and were primarily involved in cell adhesion, pathogen recognition, cellular immunity, cytokines, complement systems, and iron transport. Sixteen of the differently expressed genes involved in the immune response and the accuracy of the transcriptome data were further validated by quantitative real-time PCR (qRT-PCR). We observed Tissue sections of the spleen infected with A. hydrophila and the control group and found that the spleen of the infected group had necrosis.

Keywords: Aeromonas hydrophila; DEG; Histopathology; Immune response; Mylopharyngodon piceus; Transcriptome.

MeSH terms

Aeromonas hydrophila*
Animals
Carps / genetics*
Fish Diseases / genetics*
Fish Proteins / genetics*
Gene Expression Profiling
Gram-Negative Bacterial Infections / genetics*
Gram-Negative Bacterial Infections / pathology
Gram-Negative Bacterial Infections / veterinary
Spleen / metabolism
Spleen / pathology

Substances

Fish Proteins