Assembly of Plasmonic and Magnetic Nanoparticles with Fluorescent Silica Shell Layer for Tri-functional SERS-Magnetic-Fluorescence Probes and Its Bioapplications

Sci Rep. 2018 Sep 17;8(1):13938. doi: 10.1038/s41598-018-32044-7.

Abstract

In this study, we report on the fabrication of multilayered tri-functional magnetic-SERS-fluorescence nanoprobes (MF-SERS particles) containing clustered superparamagnetic Fe3O4 nanoparticles (NPs), silver NPs, and a fluorescent silica layer. The MF-SERS particles exhibited strong SERS signals from the silver NPs as well as both superparamagnetism and fluorescence. MF-SERS particles were uptaken by cells, allowing successful separation using an external magnetic field. SERS and fluorescence signals could be detected from the NP-containing cells, and CD44 antibody-conjugated MF-SERS particles selectively targeted MDA-MB-231 cells. Based on these properties, MF-SERS particles proved to be a useful nanoprobe for multiplex detection and separation of cancer cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line, Tumor
  • Fluorescence
  • Fluorescent Dyes / chemistry*
  • Hep G2 Cells
  • Humans
  • Hyaluronan Receptors / metabolism
  • Magnetics / methods
  • Magnetite Nanoparticles / chemistry*
  • Silicon Dioxide / chemistry*
  • Silver / chemistry
  • Spectrum Analysis, Raman / methods

Substances

  • Fluorescent Dyes
  • Hyaluronan Receptors
  • Magnetite Nanoparticles
  • colloidal silver
  • Silver
  • Silicon Dioxide