Impact of antimicrobial silver nanoparticles on anode respiring bacteria in a microbial electrolysis cell

Abstract

This study assessed the impact of antimicrobial AgNPs (50 mg L^-1, 30-50 nm) on the electrocatalytic activity of a mixed-culture anode biofilm enriched with Geobacter species. The current densities and electrochemical kinetics were maintained after exposure to AgNPs in consecutive fed-batch cycles, despite significant changes in morphological structures and bacterial communities. Bacterial community analysis showed a substantial increase in the Geobacter population in response to AgNPs exposure, indicating their higher tolerance to AgNPs. In contrast, the population of other anode respiring bacteria (ARB) belongs to Acinetobacter, Dysgonomonas, and Cloacibacillus genera appeared to be very sensitive to AgNPs toxicity as their relative abundance significantly decreased. Microscopic imaging showed that AgNPs were accumulated within anode biofilm matrix without penetration inside the cells. Moreover, the anode biofilm became denser because of enhanced extracellular polymeric substances (EPSs) production by ARB after exposure of AgNPs, implying that EPS could protect ARB against AgNPs toxicity.

Keywords: Anode respiring bacteria; Antimicrobial silver nanoparticles; Biofilm anode; Electrochemically active biofilm; Microbial electrolysis cell.