The complete mitochondrial genome of Sarcoptes scabiei var. nyctereutis from the Japanese raccoon dog: Prediction and detection of two transfer RNAs (tRNA-A and tRNA-Y)

Takafumi Ueda; Hiroshi Tarui; Nobuhide Kido; Keitaro Imaizumi; Kenji Hikosaka; Takashi Abe; Daisuke Minegishi; Yoshifumi Tada; Masataka Nakagawa; Sohei Tanaka; Tomoko Omiya; Kouki Morikaku; Minori Kawahara; Takane Kikuchi-Ueda; Teruo Akuta; Yasuo Ono

doi:10.1016/j.ygeno.2018.09.002

The complete mitochondrial genome of Sarcoptes scabiei var. nyctereutis from the Japanese raccoon dog: Prediction and detection of two transfer RNAs (tRNA-A and tRNA-Y)

Genomics. 2019 Dec;111(6):1183-1191. doi: 10.1016/j.ygeno.2018.09.002. Epub 2018 Sep 15.

Authors

Affiliations

¹ Department of Microbiology and Immunology, Teikyo University School of Medicine, 2-11-1 Kaga, Itabashi-ku, Tokyo 173-8605, Japan.
² Division of Genomic Technologies, Center for Life Science Technologies, RIKEN Yokohama Institute, 1-7-22 Suehiro-cho, Yokohama 230-0045, Japan.
³ Kanazawa Zoological Gardens, 5-15-1 Kamariyahigashi, Kanazawa-ku, Yokohama, Kanazawa 236-0042, Japan.
⁴ Department of Microbiology and Immunology, Teikyo University School of Medicine, 2-11-1 Kaga, Itabashi-ku, Tokyo 173-8605, Japan; Kyokuto Pharmaceutical Industrial Co., Ltd., 7-8 Nihonbashi Kobunacho, Chuo-ku, Tokyo 103-0024, Japan.
⁵ Department of Microbiology and Immunology, Teikyo University School of Medicine, 2-11-1 Kaga, Itabashi-ku, Tokyo 173-8605, Japan; Department of Infection and Host Defense, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8670, Japan.
⁶ Graduate School of Science and Technology, Niigata University, 8050 Ikarashi, 2-no-cho, Nishi-ku, Niigata 950-2181, Japan.
⁷ Research Institute of Bio-System Informatics, Tohoku Chemical Co., Ltd., 6-15-5, Mitake, Morioka, Iwate 020-0122, Japan.
⁸ Kyokuto Pharmaceutical Industrial Co., Ltd., 7-8 Nihonbashi Kobunacho, Chuo-ku, Tokyo 103-0024, Japan.
⁹ Department of Microbiology and Immunology, Teikyo University School of Medicine, 2-11-1 Kaga, Itabashi-ku, Tokyo 173-8605, Japan; Kyokuto Pharmaceutical Industrial Co., Ltd., 7-8 Nihonbashi Kobunacho, Chuo-ku, Tokyo 103-0024, Japan. Electronic address: t.akuta@kyokutoseiyaku.co.jp.
¹⁰ Department of Microbiology and Immunology, Teikyo University School of Medicine, 2-11-1 Kaga, Itabashi-ku, Tokyo 173-8605, Japan. Electronic address: onoy@med.teikyo-u.ac.jp.

PMID: 30223010
DOI: 10.1016/j.ygeno.2018.09.002

Abstract

Sarcoptes scabiei (Acari: Sarcoptidae) causes a common contagious skin disease that affects many mammals. Here, the complete mitochondrial genome of a mite, S. scabiei var. nyctereutis, from Japanese wild raccoon dogs was analyzed. The 13,837bp circular genome contained 13 protein-coding genes, two rRNA genes, and 22 tRNA genes. For the first time, two tRNAs (alanine and tyrosine), that were thought to be absent in scabies mites from other animals, were predicted to have short, non-cloverleaf structures by in silico annotation and detected by RT-PCR, sequencing, and northern analysis. The mitochondrial genome structure of S. scabiei is similar to that of Psoroptes cuniculi and Dermatophagoides farinae. While small and unusual tRNA genes seem to be common among acariform mites, further experimental evidence for their presence is needed. Furthermore, through an analysis of the cox1 gene, we have provided new evidence to confirm the transmission of this mite between different animal hosts.

Keywords: Mitochondrial genome; Raccoon dog; Sarcoptes scabiei; Transfer RNA.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Genome, Mitochondrial*
Phylogeny
RNA, Transfer, Ala / chemistry
RNA, Transfer, Ala / genetics*
RNA, Transfer, Tyr / chemistry
RNA, Transfer, Tyr / genetics*
Raccoon Dogs / parasitology
Sarcoptes scabiei / classification
Sarcoptes scabiei / genetics*

Substances

RNA, Transfer, Ala
RNA, Transfer, Tyr