Objective: To study the chemical constituents of the methylene chloride extract of Lonicerae Japonicae Flos.
Methods: The compounds were isolated and purified by silica gel column chromatography,Sephadex LH-20 gel column chromatography and recrystallization. The structures were elucidated by the physical and chemical properties,MS,1H-NMR and13C-NMR spectroscopy.
Results: Nine compounds were isolated and their structures were identified as β-sitosterol( 1),benzoic acid( 2),5-hydroxy-7,3’,4’-trimethoxyflavone( 3),tetrapedic acid B( 4),tricin( 5),hydnocarpin D( 6),6,7,10-trihydroxy-8-octadecenoic acid( 7),5’-methoxyhydnocarpin-D( 8) and daucosterol( 9).
Conclusion: Compound 2,4,7,8 are isolated from this plant,compounds 2,7,8 are isolated from Caprifoliaceae family for the first time.