Effect of ionic strength on bioaccumulation and toxicity of silver nanoparticles in Caenorhabditis elegans

Abstract

The behavior of silver nanoparticles (AgNPs) is influenced by environmental factors which altered their bioaccumulation and toxicity. In this study, we comprehensively investigated the influence of ionic strength on the ecotoxicity of AgNPs to Caenorhabditis elegans (C. elegans) through the transfer from Escherichia coli (E. coli). Three different exposure media (deionized water, EPA water and KM) were used to pretreat AgNPs. E. coli was then exposed to these transformed AgNPs and fed to C. elegans. Our results indicated that ionic strength significantly enhanced the reproductive toxicity (germ cell corpses, brood size and lifespan) and neurotoxicity (head trash and body bend) of AgNPs in C. elegans. Moreover, ICP-MS analysis showed that higher ionic strength increased bioaccumulation of AgNPs in E. coli and the resulting Ag body burden of E. coli affected the transfer of AgNPs to C. elegans, which might be responsible for the increased toxicity to nematodes. Furthermore, we also found that the reactive oxygen species (ROS) level in C. elegans was significantly increased after exposed to E. coli contaminated with ionic strength-treated AgNPs, which might play another important role for the enhanced toxicity of AgNPs. Overall, this study showed that the bioavailability and potential ecotoxicity of AgNPs are associated with the environmental factors.

Keywords: Bioaccumulation; Caenorhabditis elegans; Ionic strength; Silver nanoparticles; Toxicity.