In this paper, we present a phase-shifting-free method to improve the resolution of digital holographic microscopy (DHM) under the structured illumination (SI). The SI used in the system is different from the traditional SI for it is free of the visible structure due to two illumination lights with orthogonal polarization states. To separate the recorded information and also retrieve the object phase, two reference beams with different carrier frequencies and orthogonal polarization states are adopted. The principle component analysis (PCA) algorithm is introduced in the reconstruction process. It is found that the modulated frequency of SI besides the quadratic phases of the imaging system can be easily removed with help of PCA. Therefore, phase-shifting is not required both in recording and reconstruction process. The simulation is performed to validate our method, while the proposed method is applied to the resolution enhancement for amplitude-contrast and phase-contrast objects imaging in experiments. The resolution is doubled in the simulation, and it shows 78% resolution improvement in the experiments.