The aim of the study was to characterise, quantitatively and qualitatively, the yeast microbiota found during spontaneous fermentation of grape musts obtained from the red grape varieties 'Rondo' and 'Regent'. For the research, grapes originating from two vineyards (Srebrna Góra and Spotkaniówka) located in southern Poland were used. Musts of the studied grape varieties provided a favourable environment for the growth of microorganisms. Musts obtained from fruits from the Spotkaniówka vineyard were characterised by a higher overall number of yeasts. A maximum number of cells were recorded from the fourth to the ninth day of the process, in all of the analysed musts. The growing season of 2013 proved to be the least favourable for the growth of microorganisms on grapes, which resulted in their smaller number in the batches during spontaneous fermentation. During the process, succession of different groups of yeasts was observed. It was initiated by Hanseniaspora uvarum and Candida railenensis strains, which, with the progress of spontaneous fermentation, were replaced by Saccharomyces cerevisiae strains. Metschnikowia pulcherrima and Pichia membranifaciens yeasts were identified at both the beginning and the end of the process. During fermentation, Zygoascus meyerae, Kluyveromyces lactis and Nakazawaea ishiwadae strains were also identified.