Sperm cryopreservation has been widely used in assisted reproduction, but conventional techniques are not suitable for the cryopreservation of small numbers of sperm. The application of the single sperm cryopreservation technique has significantly improved the clinical treatment of cryptozoospermia and non-obstructive azoospermia. Ever since Cohen et al first developed the method of single sperm cryopreservation in 1997, constant efforts have been made to develop the carriers for this technique. In this review, we mainly discuss the existing methods and clinical outcomes of single sperm cryopreservation.
精子冷冻技术在辅助生殖支柱(ART)中已得到广泛应用，但常规冷冻方法不适用于冷冻微量精子，单精子冷冻技术的应用将在很大程度上改善隐匿性精子症、非梗阻性无精症患者的临床治疗。从1997年Cohen等最先提出单精子冷冻概念至今，研究者们不断地尝试探索单精子冷冻载体，本文依据载体的分类，对单精子冷冻方法及临床结局做一简要阐述。.
Keywords: empty zona pellucida; micro drop; testicular sperm; single sperm cryopreservation.