Objective: To explore Mycoplasma genitalium (MG) infection in the urogenital tract of infertile men and its influence on semen quality.
Methods: Semen samples were collected from 352 infertile males in the Center of Reproductive Medicine of Nanjing General Hospital from March to July 2015. MG infection was detected by real-time fluorescence simultaneous amplification and testing and semen analyses were conducted according to the WHO Laboratory Manual for the Examination and Processing of Human Semen (5th Ed) on the semen pH value, semen volume, total sperm count, sperm concentration, total sperm motility, percentages of progressively motile sperm (PMS) and immotile sperm (IMS), and sperm DNA fragmentation index (DFI). The data obtained were subjected to statistical analysis by t-test and non-parametric test (Wilcoxon test).
Results: MG infection was found in 3.4% (12/352) of the infertile patients. Compared with the MG-positive cases, the MG-negative ones showed a significantly higher semen volume (［2.85 ± 0.14］ vs ［3.84 ± 0.12］ ml, P = 0.008) and percentage of PMS (［15.86±1.72］ vs ［60.95 ± 5.63］ %, P = 0.032) but a lower DFI (［30.73 ±2.24］ vs ［20.71 ± 1.55］%, P = 0.014). However, no statistically significant differences were observed between the two groups in the semen pH value (7.38 ±0.02 vs 7.39 ± 0.01, P = 0.774), sperm concentration (［52.96 ± 15.78］ vs ［60.05 ± 4.29］×10⁶/ml, P = 0.683), sperm count (［154.15 ± 46.37］ vs ［221.56 ± 15.43］×106, P = 0.236), total sperm motility (［29.04 ± 3.11］ vs ［33.52 ± 1.51］ %, P = 0.626), or percentage of IMS (［23.57 ± 0.99］ vs ［62.34 ± 1.69］ %, P = 0.691).
Conclusions: Urogenital MG infection is common in infertile males and potentially affects the semen quality, especially sperm vitality of the patient.
目的: 探讨泌尿生殖道生殖支原体（MG）在男性不育患者中的感染情况及其对精液质量的影响。 方法： 收集2015年3月～7月来南京军区南京总医院生殖医学中心门诊的352例不育患者的精液标本。MG感染采用实时荧光核酸恒温扩增检测技术（SAT）检测，精液分析根据《WHO人类精液检查与处理实验室手册》第5版进行操作，分析精液pH值、精液量、精子总数、精子浓度、精子总活力、前向运动精子百分率（PR）、不动精子百分率（IM）、精子DNA碎片化指数（DFI）等。数据统计用t检验（t-tests）与非参数检验（Wilcoxon test）方法。 结果： 不育患者MG感染率为3.4%（12 / 352）；比较MG阳性与MG阴性不育患者间精液分析结果发现，MG阴性患者在精液量［（3.84±0.12) ml vs (2.85±0.14) ml，P=0.008］、PR［（23.57±0.99)% vs (15.86±1.72)%，P=0.032］均要显著高于 MG阳性患者，DFI［（20.71±1.55)% vs (30.73±2.24)%, P=0.014］则显著低于MG阳性患者。 而精液pH值（7.39±0.01 vs 7.38±0.02，P=0.774）、精子浓度［（60.05±4.29)×10⁶/ml vs (52.96±15.78)×10⁶/ml，P=0.683］、精子总数［（221.56±15.43)×106 vs (154.15±46.37)×106，P=0.236］、精子总活率［（33.52±1.51)% vs (29.04±3.11)%，P=0.626］、IM［（62.34±1.69)% vs (60.95±5.63)%，P=0.691］组间均无统计学差异。结论： 本研究证明：男性不育患者泌尿生殖道MG感染患者并不在少数，应引起重视；泌尿生殖道MG感染对精液质量有潜在负面影响，特别是精子活力。.
Keywords: infertility; real-time fluorescence simultaneous amplification and testing; semen quality; Mycoplasma genitalium.