Cell Cycle Analysis of the Embryonic Brain of Fluorescent Reporter Xenopus tropicalis by Flow Cytometry

Rivka Noelanders; Kris Vleminckx

doi:10.1007/978-1-4939-8784-9_17

Cell Cycle Analysis of the Embryonic Brain of Fluorescent Reporter Xenopus tropicalis by Flow Cytometry

Methods Mol Biol. 2018:1865:243-250. doi: 10.1007/978-1-4939-8784-9_17.

Authors

Rivka Noelanders^{1

2}, Kris Vleminckx^{3

4

5}

Affiliations

¹ Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium.
² Cancer Research Institute Ghent, Ghent, Belgium.
³ Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium. kris.vleminckx@ugent.be.
⁴ Cancer Research Institute Ghent, Ghent, Belgium. kris.vleminckx@ugent.be.
⁵ Center for Medical Genetics, Ghent University, Ghent, Belgium. kris.vleminckx@ugent.be.

PMID: 30151771
DOI: 10.1007/978-1-4939-8784-9_17

Abstract

Many developmental signals are associated with changes in proliferative response. Also, growing organs and tissues can contain different cellular subpopulations with a defined status in the cell cycle, e.g., quiescent in stem cells, high proliferation in progenitors, cell cycle exit in differentiating cells. This chapter describes a method for isolation of individual cell populations from the Xenopus tadpole brain and determination of their cell cycle status using flow cytometry.

Keywords: Brain; Cell cycle; Flow cytometry; Xenopus.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Brain / embryology*
Cell Cycle*
DNA / metabolism
Dissection
Embryo, Nonmammalian / cytology*
Flow Cytometry / methods*
Fluorescence
Genes, Reporter*
Permeability
Staining and Labeling
Tissue Fixation
Xenopus / embryology*

Substances

DNA